Products & Services

Dual-Labeled Probes

Precision fluorophore-quencher probes engineered for qPCR, digital PCR, SNP genotyping, and multiplex diagnostic assays.

Custom Synthesis & Ordering

Dual-Labeled Probes Ordering System

Configure your customized dual-labeled hydrolysis probe (TaqMan® style) with automated 5′ fluorophore & 3′ quencher pairing, synthesis scale, and preparative HPLC purification. Guaranteed yields are dynamically computed based on your exact sequence length.

Item Name * Min 6 alphabetic letters

Provide a unique identifier or probe name (must contain at least 6 letters, e.g. "ABCDEFG").

Enter at least 6 letters (A–Z)

Select an SKU *

Choose predefined probe SKU:

5′ Dye / 3′ Quencher

Click box to choose by dye pair:

Synthesis Scale *

Select scale of solid-phase CPG synthesis:

Enter Sequence (5′ → 3′) * 0 nt

Acceptable nucleotides: A, G, C, T only (15 to 35 nt). Characters such as 'U' are rejected automatically.

ℹ️ Enter 15 to 35 nucleotides (A, G, C, T)

Purification Services *

Dual-wavelength RP-HPLC ensures >90–95% purity. Supported SKUs offer complementary Ion-Exchange (IE-HPLC).

* Selected SKU only supports RP-HPLC.

Probe Strand Architecture

5′ 6-FAM

───

5′-AGTC...-3′

───

3′ TAMRA

Length: 0 nt | Scale: 100 nmole

Yield Delivered (Guaranteed)

Minimum Guaranteed Yield 70–80% Recovery

—

Standard recovery range; constant across chosen purification steps.

Maximum Guaranteed Yield RP-HPLC

—

Based on scale, nucleotide length, and purification method.

Selected SKU: DLP-5D1-3Q1

Dye / Quencher: 5' FAM / 3' TAM

Purification: RP-HPLC

Pricing: Quote upon request

Quantity:

Select 5′ Dye & 3′ Quencher Pair

Select a dye and quencher combination to automatically configure the corresponding SKU.

5′ Dye	Dye Code	3′ Quencher	Quencher Code	Corresponding SKU
6-FAM	D1	TAMRA	Q1	DLP-5D1-3Q1
6-FAM	D1	BHQ-1	Q2	DLP-5D1-3Q2
Cy5	D2	BBQ-650	Q3	DLP-5D2-3Q3
Cy3	D3	BHQ-1	Q2	DLP-5D3-3Q2

Multiplex Capabilities

RNA Nanorobotics Dual-Labeled Probes

RNA Nanorobotics DLP	SKU	Reporter Absorption Maximum	Reporter Emission Maximum	Quencher Type / Spectral Coverage	Common Detection Region*
6-FAM / TAMRA	DLP-5D1-3Q1	495 nm	520 nm	TAMRA fluorescent acceptor/quencher; Abs ~559 nm, Em ~583 nm	FAM / Green
6-FAM / BHQ-1	DLP-5D1-3Q2	495 nm	520 nm	BHQ-1 dark quencher; λmax ~534 nm; strong absorption ~480–580 nm	FAM / Green
Cy3 / BHQ-1	DLP-5D3-3Q2	550 nm	564 nm	BHQ-1 dark quencher; λmax ~534 nm; strong absorption ~480–580 nm	Cy3 / Yellow-Orange
Cy5 / BBQ-650	DLP-5D2-3Q3	648 nm	668 nm	BBQ-650 dark quencher; λmax ~650 nm; useful absorbance ~550–750 nm	Cy5 / Far-Red

*Actual channel names and optical filter sets vary by qPCR instrument; customers should confirm compatibility with their specific platform.

Dual-Labeled Probes