RNA Instruments & Capabilities

Specifications

• •Synthesis Method: Solid-phase phosphoramidite chemistry
• •Supported Oligonucleotides: Chemically modified RNA
• •Amidite Capacity: 20 amidite positions standard (configurable up to 24)
• •Ancillary Reagents: Supports up to 9 ancillary reagents
• •Backbone Chemistry: Phosphodiester and phosphorothioate linkages, including mixed-backbones
• •Modified Chemistry: Standard & modified phosphoramidites (2′-F, fluorophores, affinity tags, reactive groups)

Application

The MerMade 48X is an automated oligonucleotide synthesizer designed for high-throughput, parallel synthesis of modified RNA using standard and modified phosphoramidite chemistry. MerMade 48X is able to synthesize up to 48 individual oligonucleotide strands in a single run, enabling efficient production of highly customizable RNA strands with different sequences, lengths, and chemical modifications.

Supports the incorporation of modified nucleotides such as 2′-F and other compatible modified phosphoramidites, as well as fluorophores, affinity tags, and functional groups for downstream labeling, conjugation, and bioconjugation applications.

Specifications

Application

The Bio-Rad PowerPac Basic–powered gel electrophoresis system provides controlled electrophoretic separation for the characterization of RNA or modified RNA. Gel electrophoresis is used as a complementary analytical method to evaluate RNA integrity, approximate size, purity, product homogeneity, and the presence of truncated or higher-molecular-weight species. Depending on the analytical objective and gel configuration, native or denaturing polyacrylamide gel electrophoresis (PAGE) may be used for characterization of synthesized RNA and modified oligonucleotides.

Specifications

• •Camera Resolution: 5.4-megapixel scientific imaging detector
• •Image Bit Depth: 16-bit, 65,536 grayscale levels for high dynamic range
• •Field of View: 20 × 15 cm
• •UV Excitation: Dual-wavelength UV transilluminator (302 nm & 365 nm)
• •Blue-Light Excitation: Epi blue LED illumination for sensitive fluorescent nucleic acid dyes
• •White-Light & Color: Transmitted white light & color imaging supported

Application

The Azure Biosystems Azure 200 Gel Imaging System is a high-resolution digital imaging platform designed for visualization, documentation, and analysis of stained nucleic acids. With multiple illumination modes, including ultraviolet, blue light, and white light, the system enables sensitive detection of RNA and oligonucleotide bands following gel electrophoresis. Azure 200 is used to evaluate RNA integrity, approximate size, product homogeneity, and the presence of truncated or higher-molecular-weight species.

Specifications

• •HPLC Application: Preparative HPLC
• •Pump Configuration: Gilson 333 master pump with Gilson 334 secondary pump
• •Flow Rate Range: 1–200 mL/min (up to 400 mL/min with parallel pumps)
• •Maximum Pressure: 3,040 psi (210 bar)
• •Loading Capacity: Milligram-to-gram scale, up to ~15 g depending on column dimensions
• •Software Control: TRILUTION® LC Software for method control, gradient programming, detection & fraction collection

Application

The Gilson VERITY 281 Preparative HPLC System is designed for preparative chromatographic purification with automated sample injection and fraction collection. For RNA applications, the system can be configured with an appropriate chromatographic column and mobile-phase system for purification of synthetic RNA and chemically modified oligonucleotides.

The system is suitable for ion-pair reversed-phase HPLC and other compatible chromatographic methods for separation of the desired full-length RNA product from synthesis-related impurities, truncated sequences, and other process-related components. The combination of the 333/334 pumps provides controlled solvent delivery and gradient formation, while the GX-281 Liquid Handler enables automated sample introduction and collection of purified fractions.

Specifications

• •Sample Volume: 0.5–5 µL applied directly to the hydrophobic sample plate
• •Wavelength Scan Range: 200–900 nm
• •Optical System: Xenon light source; 0.2 mm and 0.5 mm pathlengths with automatic pathlength selection
• •Nucleic Acid Analysis: Rapid concentration measurement and purity ratios (A260/A280 and A260/A230)

Application

The GE Healthcare NanoVue Plus is a stand-alone microvolume UV/Visible spectrophotometer for rapid quantification and quality assessment of RNA or oligonucleotides. It enables measurement of RNA or oligonucleotide concentration and evaluates absorbance profiles during synthesis, purification, conjugation, and characterization.

The instrument is particularly useful for routine quality control before downstream applications such as electrophoresis, chromatography, enzymatic reactions, and other RNA analysis procedures.

Specifications

• •Concentration Method: Centrifugal vacuum concentration
• •Drive Speed: 1,725 rpm; approximately 300 × g centrifugal force
• •Drying Rates: LOW (ambient), MEDIUM (43°C), and HIGH (65°C)
• •Vacuum Pump: Integral oil-free diaphragm pump
• •Vacuum Level: 7 torr (9 mbar)
• •Rotor Formats: 24 × 1.5 mL, 36 × 1.5 mL, 48 × 0.5 mL, 72 × 0.5 mL tubes
• •Timer & Modes: 5-min increments up to 9h 55m; Manual or Automatic modes

Application

The Thermo Scientific Savant DNA120 SpeedVac Concentrator is a centrifugal vacuum concentration system designed for drying low-volume RNA samples. The system combines centrifugal force and vacuum for bump-free sample drying. An integrated chamber heater counteracts evaporative cooling and accelerates solvent evaporation, with selectable drying conditions at ambient temperature, 43°C, or 65°C.

Specifications

• •Sample Capacity: 96 wells with 0.2 mL sample block
• •Reaction Volume: 10–100 µL per well
• •Temperature Range: 4–99.9°C with calibration traceable to NIST
• •VeriFlex™ Technology: 6 independently controlled temperature zones across the 96-well block (0.1°C to 5.0°C differences between adjacent zones)
• •Heated Cover: Provides uniform pressure and minimizes condensation during thermal cycling

Application

The Applied Biosystems Veriti™ 96-Well Thermal Cycler is used for controlled thermal cycling in PCR and other temperature-dependent nucleic acid workflows. VeriFlex™ technology provides six independently controlled temperature zones across the 96-well block, allowing optimization of thermal cycling conditions such as annealing temperature within a single run.

Specifications

• •Chamber Capacity: 34 L (1.2 ft³)
• •Sterilization Temp: Up to 135°C
• •Liquid Sterilization: Dedicated 121°C cycle for compatible media, buffers, and liquids
• •Material Compatibility: Autoclavable glassware, surgical/prep metals, heat-resistant polymers
• •Safety Features: Pressure monitoring, door interlock protection, and auto-pressure relief

Application

The Thermo Scientific SterileMax Series 1277 is a benchtop steam sterilizer designed for sterilization of laboratory materials using controlled high-temperature steam cycles. Used to sterilize compatible reusable laboratory tools, glassware, containers, and media before RNA preparation and nanostructure assembly.