Protein Instruments & Capabilities

Specifications

• •Chromatography: Analytical and small-scale preparative size-exclusion chromatography (SEC)
• •Sample Volume: 20–500 µL, depending on column configuration
• •Sample Injection: Manual V9-Inj injection valve; compatible with Alias autosampler
• •UV Detection: Compatible with U9-M and U9-L UV monitors
• •Fraction Collection: Compatible with F9-R fraction collector
• •System Control: UNICORN software for method control, pressure limits, automated sample loading & fraction collection

Application

The ÄKTA pure 25 is a flexible chromatography system designed for analytical and small-scale preparative protein purification by size-exclusion chromatography. The system can be configured with low-volume flow paths to minimize sample dilution and improve chromatographic resolution, making it suitable for separation and analysis of proteins and other biomolecules based on molecular size.

It supports both manual and automated sample injection, UV-based detection, and fraction collection for recovery of purified sample fractions with high biological activity.

Specifications

• •Speed Range: 1,000 to 80,000 rpm, adjustable in 100 rpm increments
• •Speed Control: ±50 rpm of set speed above 1,000 rpm
• •Temperature Range: 0 to 40°C with ±1°C precision control
• •Run Time: Up to 99 hours 59 minutes, continuous HOLD mode available
• •Vacuum System: Reduces chamber pressure to below 5 microns (0.7 Pa)

Application

The Beckman Coulter Optima LE-80K is a high-speed preparative ultracentrifuge designed for controlled separation and recovery of biological and biochemical samples. For protein-related applications, the system supports purification and fractionation workflows involving proteins, protein complexes, and other macromolecular components.

Centrifugation conditions can be adjusted according to sample and separation requirements, including rotor speed, temperature, run time, and acceleration or deceleration profile. Sample volume and loading capacity depend on the selected rotor and tube configuration. Integrated overspeed and rotor-energy systems provide additional safeguards during high-speed runs.

Specifications

• •Shaking Speed: 25–300 rpm for a two-unit stacked configuration
• •Speed Control Accuracy: ±1 rpm
• •Temperature Range: 10°C above ambient to 80°C
• •Sample Capacity: Supports Erlenmeyer flasks up to 5 L, tubes, and plates, with maximum total platform load of 15.5 kg (34 lb)
• •Operation Modes: Continuous, timed up to 99.9 hours, and programmable operation

Application

The New Brunswick Innova 44 dual-stack incubator shaker provides controlled agitation and temperature for biological solutions and cultures in reaction vessels. For protein production workflows, it supports culture growth and expansion prior to downstream protein recovery and purification.

The dual-stack configuration increases available culture capacity while accommodating a variety of flasks, tubes, and plates, making it suitable for routine protein production and process development applications. Continuous, timed, and programmable operation allows shaking speed and temperature conditions to be adjusted according to the requirements of the culture or process.

Specifications

• •Chamber Capacity: 190 L
• •Shaking Speed: 30–300 rpm
• •Temperature Range: 4–60 °C
• •CO₂ Control Range: 0–20% CO₂ regulation
• •Timer & Load: Up to 999 hours 59 minutes; maximum load capacity 10 kg
• •Programmable Operation: Supports up to 12 sequential steps with adjustable temperature and shaking speed

Application

The VWR® 190 L Compact Magnetic Drive CO₂ Incubator Shaker provides a controlled environment for cultivation of biological systems requiring simultaneous agitation, temperature regulation, humidity, and CO₂ control. It is suitable for protein-production workflows involving suspension cell culture, microbial culture, recombinant protein expression, and other upstream bioprocessing applications where consistent mixing and environmental control are required.

The system can accommodate a range of flask, tube-rack, microplate, and other culture-vessel configurations, making it suitable as a flexible upstream protein-production platform.

Specifications

• •Frequency: 20–25 kHz
• •Power: 1.5–150 W, continuously adjustable
• •Processing Volume: 0.1–150 mL
• •Program Memory: Stores and recalls up to 20 sonication programs
• •Standard Horn: 6 mm titanium horn, suitable for sample volumes of 10–100 mL

Application

The Benchmark Scientific Pulse 150™ Ultrasonic Homogenizer uses ultrasonic waves and cavitation for controlled cell disruption and sample homogenization. For protein-related workflows, the system can be used to disrupt cells and prepare samples for downstream protein extraction, analysis, or purification.

Adjustable power and programmable pulsed-mode operation provide flexibility for different sample volumes and processing requirements. The system is also designed for DNA shearing and sample preparation for ChIP assays.

Specifications

Application

The Bio-Rad PowerPac Basic–powered gel electrophoresis system provides controlled electrophoretic separation for proteins. For protein analysis, the system supports polyacrylamide gel electrophoresis (SDS-PAGE / native PAGE) for separation and evaluation of protein samples according to molecular size or electrophoretic mobility.

Specifications

• •Camera Resolution: 5.4-megapixel imaging detector
• •Image Bit Depth: 16-bit, 65,536 grayscale levels
• •Field of View: 20 × 15 cm
• •UV Excitation: Dual-wavelength UV transilluminator (302 nm & 365 nm)
• •Blue-Light Excitation: Epi blue LED illumination for compatible fluorescent dyes
• •White-Light Imaging: Transmitted white light for colorimetric protein stains
• •Color Imaging: Full color documentation supported

Application

The Azure Biosystems Azure 200 Gel Imaging System is a high-resolution digital imaging platform designed for visualization, documentation, and analysis of stained protein gels. Its integrated imaging workflow provides reproducible digital gel images suitable for analytical documentation and comparative sample assessment.